Insecticide resistance in Anopheles gambiae sensu lato (Diptera: Culicidae)  across different agroecosystems in Niamey, Niger

Noura Mamane Salé1,2*; Rabiou Labbo; Ibrahim Maman Laminou; Ibrahima Issa Arzika2; Ali Djibo Souley; Wilfried Zoulkifouly Hounkarin; Halima Zamanka Naroua; Amadou  Soumana2 ; Abdoul-Aziz Maiga3; Ronan Jambou2,4 & Ali Doumma


Malaria vector control in Niger is currently based on the distribution of insecticide treated nets. However, vectors resistance to insecticides represents a major threat to the current national strategy against malaria. This study aims to characterize the impact of agroecosystems on insecticide resistance in Anopheles gambiae s.l. at Niamey. Larvae collected were reared until emergence. Adults aged 2-5 days were used to assess susceptibility to insecticides (pyrethroids, DDT and bendiocarb) after pre-exposure to piperonyl butoxide (PBO) synergist according to WHO protocols. PCRs were performed to identify the sibling species of An. gambiae complex and characterization resistance mutations (Kdr and ace-1). Overall, An. gambiae s.l. was resistance to pyrethroids and DDT (mortality rates from 1% to 55%) and susceptible to bendiocarb at most sites. Pre-exposure to the PBO synergist resulted in partial restoration of pyrethroid susceptibility. Two species of An. gambiae complex were found: An. arabiensis and An. coluzzii. The presence of An. coluzzii was strongly correlated with agricultural practices (99% in rice cultivation sites). Kdr mutations were found at all sites with kdr-w ranging from 45% to 70% in mosquitoes collected in unirrigated and rice field, respectively, and kdr-e found at 37% to 47% at each type of site, respectively. The ace-1 mutation was detected at low frequency (1%) and only from two rice cultivation sites. The high levels of pyrethroid and DDT resistance detected in Niamey had a strong link with rice cultivation, shown that agriculture is a driver of resistance that can compromise control malaria efforts.

Keywords: Anopheles gambiae sensu lato, insecticide, resistance, agroecosystem, vector control, Niamey, Niger..


1Faculté des Sciences et Techniques, Université Abdou Moumouni de Niamey, Niger

2Unité Paludologie et Entomologie Médicale, Centre de Recherche Médicale et Sanitaire, BP10887, Niamey Niger

3Université Joseph KI-ZARBO, Ouagadougou, Burkina Faso

4Département Santé Globale, Institut Pasteur, Paris, France

Infos article

African Journal of Tropical Entomology Research: 3 (1) pp. 30-40

© 2024, Noura et al.


*Corresponding author: [email protected]

Received: 05 January 2024

Accepted: 27 April 2024

Published : 02 May 2024

Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution 4.0 International license.

Citation : Mamane Salé N.; Labbo R. ; Maman Laminou I.; Issa Arzika I.; Djibo Souley A. ; Zoulkifouly Hounkarin W. ; Zamanka Naroua H.; Soumana A.;  Maiga A.; Jambou R. & Doumma A(2024). Insecticide resistance in Anopheles gambiae sensu lato (Diptera: Culicidae)  across different agroecosystems in Niamey, Niger. African journal of tropical entomology research, 3(1), 30-40.


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